sexta-feira, 7 de outubro de 2011

Toxicity of the Fludioxonil and Cyprodinil preparation

Like i said, the usage of this preparation follows a protocol wich i have developed after consecutive trials with newly inoculated explants, as well as, after experimenting a little with non contaminated explants to evaluate plant toxicity.
Acute toxicity seems to affect only very small and sensitive portions of tissue or explants, but so far i have only exposed, Ilex , Gardenia, Rose, Petunia and Dianthus to these substances. Asside from that there is the question of the solvent wich is Cetone, and this product by itself might carry some harmfull properties to the culture medium. My conslusion for Olea europaea was that the toxicity afects only uninodal explants in my most comon concentration of 1ml of the concentrate per liter of culture medium. Explants rescued from the treatment 2 or 3 weeks after inoculation, can be recovered clear of fungus except some white moulds, specially filamentous ones ( similar to cotton candy).
Here we see explants tretaed with cyp.& flu. antifungal preparation.
Rifampicin here like with PPM seems to greatly reduce the chlorosis phenomena on the treated explants. 

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