domingo, 30 de outubro de 2011

Phormium tenax in vitro

 Fase 1 – Establishment. Cultures have been established of Phormium tenax in vitro from seed and a new method for induction of high germination rates has been discovered. For in vitro propagation seeds need to be isolated from desinfected unopened seed pods  and inoculated in Antifungal containing medium, after mild desinfection. Establishment of healthy cultures has been achieved but inicial trials, left only few live growing material , nevertheless   new trials from 2010 summer seeds will bring abundant cultures since media and growth regulator concentrations have recently been adjusted with success.
Fase 2 – Propagation. Like for the establishment fase, significant progress has been achieved with Phormium tenax proliferation. Moreover our cultures show no loss of the varieagated properties of the mother plant, wich has been mentioned as being a bottleneck for in vitro propagation of the variegated forms of phormium. Growth regulator inbalance is still in test for even better growth rates.


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