The use of PPM in the culture medium against the fungal contaminations was inefective.
Contaminations were coming back after 3 weeks of culture, and the subsequent fungal colonies seemed to be unafected by PPM dosage, wich was about 2ml per liter of culture medium.
I had still my secret weapon to test against this problem.
Like i described before, it consists of extracting Cyprodinil and Fludioxonil from a comercial phytosanitary product. Please be so kind to ask for detailed process of elaboration of the solution....I have briefly described it in a previous post.
So what are these antifungal agents?
Fludioxonil is an analogue synthetic molecule based on the natural compound pyrrolnitrin produced by the soil-borne bacterium Pseudomonas pyrrocinia. Chemists had to find a similar molecule with a more stable behaviougr since pyrrolnitrin was very sensitive to light. Find more here: https://docs.google.com/viewer?a=v&q=cache:CKjtrEL0blYJ:www.farmassist.com/postharvest/images/GraduateTechBroFinal.pdf+fludioxonil+pseudomonas+pyrrocinia&hl=en&pid=bl&srcid=ADGEESiM6VmOAKqhw5soFVwAQTlEfK4E8t2iNTxtVYs1hi0F4SyVQA9zsFMVaLk7Cri-5crbTtqXmTvsDpt9JhzN2kFVO9-ePR1EOOtDK123bEfW1ypP343W_tTMSYwvTO9JOfAAWVE_&sig=AHIEtbSwxwnu6J9lXOOJeWm56ghW_oWj3w Fludioxonil is considered a low human risk compound.
Cyprodinil is a synthetic compound discovered when analising the anti-fungal activity of a range of sulfonylureas. It is a systemic substance with a broad spectrum activity for a number of plant diseases, including Botrytis cinarea. Find more here:http://pubs.acs.org/doi/abs/10.1021/bk-1998-0686.ch024
In terms of safety Cyprodinil is untill further notice a relatively safe substance to humans, and generally safe for nature. Its most acute toxicity is by contact and inalation. Persistence in the soil has been demonstrated but mobility is very low. The most harmfull consequences for cyprodinil are for aquatic ecossystems, having very high impacts even in small dosis, nevertheless its poor mobility makes is reasonably safe as long as simple precautions are taken.
So what are these antifungal agents?
Fludioxonil is an analogue synthetic molecule based on the natural compound pyrrolnitrin produced by the soil-borne bacterium Pseudomonas pyrrocinia. Chemists had to find a similar molecule with a more stable behaviougr since pyrrolnitrin was very sensitive to light. Find more here: https://docs.google.com/viewer?a=v&q=cache:CKjtrEL0blYJ:www.farmassist.com/postharvest/images/GraduateTechBroFinal.pdf+fludioxonil+pseudomonas+pyrrocinia&hl=en&pid=bl&srcid=ADGEESiM6VmOAKqhw5soFVwAQTlEfK4E8t2iNTxtVYs1hi0F4SyVQA9zsFMVaLk7Cri-5crbTtqXmTvsDpt9JhzN2kFVO9-ePR1EOOtDK123bEfW1ypP343W_tTMSYwvTO9JOfAAWVE_&sig=AHIEtbSwxwnu6J9lXOOJeWm56ghW_oWj3w Fludioxonil is considered a low human risk compound.
Cyprodinil is a synthetic compound discovered when analising the anti-fungal activity of a range of sulfonylureas. It is a systemic substance with a broad spectrum activity for a number of plant diseases, including Botrytis cinarea. Find more here:http://pubs.acs.org/doi/abs/10.1021/bk-1998-0686.ch024
In terms of safety Cyprodinil is untill further notice a relatively safe substance to humans, and generally safe for nature. Its most acute toxicity is by contact and inalation. Persistence in the soil has been demonstrated but mobility is very low. The most harmfull consequences for cyprodinil are for aquatic ecossystems, having very high impacts even in small dosis, nevertheless its poor mobility makes is reasonably safe as long as simple precautions are taken.
Sem comentários:
Enviar um comentário