Fase 1 and 2 – The establishment fase and culture medium used for Dianthus has been very similar to the ones used for Petunias, in fact the first trials with these herbaceous species were taken using Dianthus seeds. The cultures have very similar needs but besides vitrification Dianthus has also a tendency for whitening after the third week of culture. All other phenomena like callus production,and secondary embriogenesis, follows a very similar pattern for both species.
We have currently 3 species of dianthus growing in vitro in our culture room, D caryophilus , D. barbatus and D. chinensis cv Chianty. The next step with these cultures will be adjustment of salt concentrations to allow longer periods before medium transfer operations, and better overall robustness of explants since propagation rates are more than acceptable.
We have currently 3 species of dianthus growing in vitro in our culture room, D caryophilus , D. barbatus and D. chinensis cv Chianty. The next step with these cultures will be adjustment of salt concentrations to allow longer periods before medium transfer operations, and better overall robustness of explants since propagation rates are more than acceptable.
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