Rifampicin a possible nitrogen carrier for Olea tissue culture

That's right, but before I go on explaining why i think that Rifampicin might work as a nitrogen carrier let me tell you how i got there.
Like i was saying just a couple of messages before, my last year of work as been mostly devoted to understand why it wasn't possible to scale up the production of the Galega clones at the lab where i was working.
If you were following me from there, you know that my first preocupation was to "close all the windows at the LAB". In other words, increasing the level of certainty in all asseptic procedures, making sure our error or errors wern't having aditional help in spreading the contaminations through the all culture stock. It is important to say that it takes some time to do this. Firstly it is necessary to repeat several cycles of multiplication, and it's related smaller procedures, like medium elaboration, labware cleaning, infrastrucure and equipment cleaning and desinfection, etc, just to understand how rich the culture medium that you are using is for the type of organisms that you are up against, and how vulnerable your actions may reflect in the transfering operations and culture medium distribution.
To simply get the hang of it 6 months would be an acceptable time frame, specially being olive a slow growth species.- I didn't have such time.- This company was thirsty for results and involved in a chronic lack of faith by it's partners and investors.

-After the first statistical surveys on the contamination patterns, it was very obvious that the total impact of fungus was the biggest hazzard for the lab. This was proven by evidence that fungus were continuously making theyr appearence throughout the all process of propagation, wich from early stages, led me to believe that the spores were coming either from the explants and having diferenciated responses along the time of culture, allowing a continuous but  slow germination of spores after first contact with the culture medium. Or simply coming from the outside of the culture vessels in a randomly fashion along time.
Even so:
Statistical results after 3 months were somewhat contradictory.
Changes introduced in several laboratory procedures were still young to give any concrete credible results.
There was a strong conviction of my supervisor that problems were coming from the transfer operations.

After testing PPM (plant preservative mixture) in the cultures, the same antibiotic wich had recently given me  promissing results in the establishment fase for Madural cultivar at my own Lab, it proved ineficient against the fungal contaminations in the Galega stock.
 - It's worth mentioning that PPM has reasonable eficiency against fungus, but it's degradation in the medium along time, makes way for late contaminations. This is what happened basically. After 3 to 4 weeks fungus were back, and since we had a hard time keeping cultures up to date, the situation turned persistently to the same status quo. Continuous loss of explant material.